Fun Fact: What is so special about an odd k-mer length?
by: onestop_data
Have you noticed that most assemblers use an odd k-mer length? Do you know why? Don’t you think it is odd? This blog post explains below …
Painless Random Forest Regression in Python – Step-by-Step with Sklearn
by: onestop_data
This tutorial demonstrates a step-by-step on how to use the Sklearn Python Random Forest package to create a regression model.
Insights on a Plethora of Python IDEs and Code Editors
by: onestop_data
Python is a powerful, easy to use, general-purpose programming language, loved by most programmers to develop web applications, data science, software prototypes, etc. Being a widely …
Estimate Genome Size and Best k-mer Size for Assembly – Step-by-Step
by: onestop_data
This tutorial shows how you can estimate the genome size and the best k-mer length for genome assembly using KmerGenie. Moreover, the tutorial shows how to …
Reference-Free Metagenomic Datasets Comparison – Step-by-Step
by: onestop_data
Reference-free metagenomic methods are very useful to compare datasets with high levels of unknown sequences. This tutorial teaches how this can be done going from metagenomic …
Bloom Filter Made Simple: Theory and Code
by: onestop_data
This tutorial simplifies Bloom Filter in Python by teaching what is a bloom filter, talks about its false positive and false negative rate, introduces some graphics …
Painless Prokaryote Pan Genome – Step-by-Step
by: onestop_data
This tutorial shows how to annotate genomes in FASTA format and how to generate the pan-genome and core genome using the annotation. For annotating the genes, …
How to Simulate NGS reads – Step-by-Step
by: onestop_data
This tutorial shows how to use a read simulator in Next-Generation Sequencing (NGS) sequence for single-cell genomes and metagenomics. It covers how to simulate Illumina (NovaSeq, …
Genome Assembly for Omics – Step-by-Step
by: onestop_data
This tutorial will show step-by-step how to assembly single-cell Microbial genomes and metagenomic datasets. Here we will be using SPAdes to assemble the datasets and show …
Painless Metagenomic Contigs Binning – Step-by-Step
by: onestop_data
This blog post teaches a step-by-step on binning metagenomic contigs using two unsupervised methods – CONCOCT and Metabat2. Furthermore, it uses a simulated metagenomic to evaluate …