Unix

The Simplest Way to Terminate a Unix Screen Session

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This tutorial shows how to terminate a Unix screen session. This can be accomplished most of the time by calling the exist command; however, the command …

Machine Learning

Painless Kmeans in Python – Step-by-Step with Sklearn

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This tutorial shows how to use k-means clustering in Python using Scikit-Learn which can be installed using bioconda.

Bioinformatics

Calculate DNA Melting Temperature in Python – Step-by-Step

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This tutorial shows how to calculate DNA melting temperature (Tm) in Python using Biopython which can be installed using bioconda.

Bioinformatics

Masking Low Complexity Regions – Step-by-Step

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This short tutorial teaches how to mask low complexity regions in a FASTA file. This can be quickly accomplished by using bbmap which can download using …

Machine LearningPython

Simple R Squared Calculation in Python

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This short tutorial shows how to find the R squared value in Python using sklearn which can be useful when looking at the data correlation in …

Bioinformatics

Fast FASTA/FASTQ Random Subsampling

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This short tutorial teaches how to subsample a paired FASTQ, single FASTQ, pair FASTA, or single FASTA file to a specific number of reads. This can …

Data visualization

Heatmap Plotting in Python – The Simplest Way

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This short tutorial teaches how to use Python seaborn which relies on matplotlib to plot a heatmap the simplest way.

Bioinformatics

Easy NCBI Genome Download

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NCBI genome download can be a very unpleasant job. This page shows how to use ncbi-genome-download to easily download NCBI genomes with a single command line.

Unix

Find All Files Containing a Specific Text on Linux or macOS

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This short tutorial teaches you how to find all files containing a specific text on linux or macOS using either the command find or grep. Find …

Bioinformatics

Fast Conversion of Lowercase Sequences to Uppercase in FASTA Format

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This tutorial teaches two approaches to convert lowercase to uppercase sequences in FASTA format. In bioinformatics, a FASTA with lowercase bases implies that regions are low …