Bioinformatics

Creating a Multiple Sequence Alignment (MSA) Aware of Forward and Reverse Complement Directions – Easy

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This blog post teaches an easy way on how to create a multiple sequence alignment (MSA) aware of forward and reverse complement directions.

Python

Upload Python Package to PyPi – Step-by-Step

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This tutorial will teach you how to upload a Python Package to PyPi and show all the requirements needs to get this done.

BioinformaticsUnix

How to Create a RamDisk on Linux – Step-by-Step

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This tutorial will teach you how to create a ramdisk on a Linux distribution, and how to unmount it when needed. This can be used to …

Docker

How to Backup a Docker Container and Restore it – Step-by-Step

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Here, you will learn how to backup and restore a Docker container locally – an alternative approach to push and pull the container from Docker Hub. …

Unix

The Simplest Way to Terminate a Unix Screen Session

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This tutorial shows how to terminate a Unix screen session. This can be accomplished most of the time by calling the exist command; however, the command …

Machine Learning

Painless Kmeans in Python – Step-by-Step with Sklearn

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This tutorial shows how to use k-means clustering in Python using Scikit-Learn which can be installed using bioconda.

Bioinformatics

Calculate DNA Melting Temperature in Python – Step-by-Step

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This tutorial shows how to calculate DNA melting temperature (Tm) in Python using Biopython which can be installed using bioconda.

Bioinformatics

Masking Low Complexity Regions – Step-by-Step

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This short tutorial teaches how to mask low complexity regions in a FASTA file. This can be quickly accomplished by using bbmap which can download using …

Machine LearningPython

Simple R Squared Calculation in Python

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This short tutorial shows how to find the R squared value in Python using sklearn which can be useful when looking at the data correlation in …

Bioinformatics

Fast FASTA/FASTQ Random Subsampling

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This short tutorial teaches how to subsample a paired FASTQ, single FASTQ, pair FASTA, or single FASTA file to a specific number of reads. This can …