Creating a Multiple Sequence Alignment (MSA) Aware of Forward and Reverse Complement Directions – Easy
This blog post teaches an easy way how to create a multiple sequence alignment (MSA) aware of forward and reverse complement directions.
How to Create a RamDisk on Linux – Step-by-Step
This tutorial will teach you how to create a ram disk on a Linux distribution and unmount it when needed. This lesson can be used to …
Calculate DNA Melting Temperature in Python – Step-by-Step
This tutorial shows how to calculate DNA melting temperature (Tm) in Python using Biopython, installed using bioconda.
Masking Low Complexity Regions – Step-by-Step
This tutorial teaches how to mask low complexity regions in a FASTA file using BBMap.
Fast FASTA/FASTQ Random Subsampling
This short tutorial teaches how to subsample a paired FASTQ, single FASTQ, pair FASTA, or single FASTA file to a specific number of reads. This can …
Easy NCBI Genome Download
NCBI genome download can be a very unpleasant job. This page shows how to use NCBI-genome-download to download NCBI genomes with a single command line easily.
Fast Conversion of Lowercase Sequences to Uppercase in FASTA Format
This tutorial teaches two approaches to convert lowercase to uppercase sequences in FASTA format. In bioinformatics, a FASTA with lowercase bases implies that regions are low …
Quick Sequencing Depth and Coverage Check
This tutorial teaches how to run a quick sequencing depth and coverage check for one or more reference sequences.
The Fastest Way to Read a FASTA in Python
This tutorial teaches a fast approach to how to read sequences from large FASTA files in Python using Pysam. I know how reading large FASTA files …
Multiple Sequence Alignment – Theory and Practice – Step-by-Step
This blog post described Multiple Sequence Alignment (MSA) focusing on the theory and practice – Step-by-Step using MAFFT and Muscle.