The Easiest Way to Download Genomic Data from NCBI SRA, MG-RAST, etc
This tutorial will teach you how to download NGS data and metadata from repositories such as NCBI SRA, MG-RAST, Imicrobe, etc – very helpful to download …
Fun Fact: What is so special about an odd k-mer length?
Have you noticed that most assemblers use an odd k-mer length? Do you know why? Don’t you think it is odd? This blog post explains below …
Estimate Genome Size and Best k-mer Size for Assembly – Step-by-Step
This tutorial shows how you can estimate the genome size and the best k-mer length for genome assembly using KmerGenie. Moreover, the tutorial shows how to …
Painless Prokaryote Pan Genome – Step-by-Step
This tutorial shows how to annotate genomes in FASTA format and how to generate the pan-genome and core genome using the annotation. For annotating the genes, …
How to Simulate NGS reads – Step-by-Step
This tutorial shows how to use a read simulator in Next-Generation Sequencing (NGS) sequence for single-cell genomes and metagenomics. It covers how to simulate Illumina (NovaSeq, …
Everything About the NCBI BLAST Aligner and Faster Alternatives
This blog post talks about everything you need to know about BLAST (Basic Local Alignment Search Tool) NCBI. Here you will learn: How to run it …
Scaffolding Genome: Increase Draft Genome N50 Length – Step-by-Step
This tutorial demonstrates how to increase a draft genome N50 by using Scaffold_Builder which takes pre-assembled genomes and a closely related reference genome.