The Easiest Way to Download Genomic Data from NCBI SRA, MG-RAST, etc
by: onestop_data
This tutorial will teach you how to download NGS data and metadata from repositories such as NCBI SRA, MG-RAST, Imicrobe, etc – very helpful to download …
Fun Fact: What is so special about an odd k-mer length?
by: onestop_data
Have you noticed that most assemblers use an odd k-mer length? Do you know why? Don’t you think it is odd? This blog post explains below …
Estimate Genome Size and Best k-mer Size for Assembly – Step-by-Step
by: onestop_data
This tutorial shows how you can estimate the genome size and the best k-mer length for genome assembly using KmerGenie. Moreover, the tutorial shows how to …
Reference-Free Metagenomic Datasets Comparison – Step-by-Step
by: onestop_data
Reference-free metagenomic methods are very useful to compare datasets with high levels of unknown sequences. This tutorial teaches how this can be done going from metagenomic …
Painless Prokaryote Pan Genome – Step-by-Step
by: onestop_data
This tutorial shows how to annotate genomes in FASTA format and how to generate the pan-genome and core genome using the annotation. For annotating the genes, …
How to Simulate NGS reads – Step-by-Step
by: onestop_data
This tutorial shows how to use a read simulator in Next-Generation Sequencing (NGS) sequence for single-cell genomes and metagenomics. It covers how to simulate Illumina (NovaSeq, …
Genome Assembly for Omics – Step-by-Step
by: onestop_data
This tutorial will show step-by-step how to assembly single-cell Microbial genomes and metagenomic datasets. Here we will be using SPAdes to assemble the datasets and show …
Painless Metagenomic Contigs Binning – Step-by-Step
by: onestop_data
This blog post teaches a step-by-step on binning metagenomic contigs using two unsupervised methods – CONCOCT and Metabat2. Furthermore, it uses a simulated metagenomic to evaluate …
Everything About the NCBI BLAST Aligner and Faster Alternatives
by: onestop_data
This blog post talks about everything you need to know about BLAST (Basic Local Alignment Search Tool) NCBI. Here you will learn: How to run it …
Scaffolding Genome: Increase Draft Genome N50 Length – Step-by-Step
by: onestop_data
This tutorial demonstrates how to increase a draft genome N50 by using Scaffold_Builder which takes pre-assembled genomes and a closely related reference genome.